Fig. 1.

The glycometabolic labeling performance of azido-or BCN-sugar in active CAR-T and tumor cells, respectively. (a) The scheme of artificial metabolic labeling for CAR-T and tumor cells. (b–e) Confocal imaging and flow cytometry analyses of CAR-T and Raji cells separately stained with DBCO-Fluor488 and N₃-Cy5.5 for 30 min after incubation with Ac₄GalNAz and Ac₄ManNBCN, respectively. Scale bar = 10 μm. (f–h) Flow cytometry analyses of the stability of BCN group on Raji cell in vitro and in vivo from day 0 to day 6 post labeling of BCN groups using Ac₄ManNBCN. (i–j) The generation of BCN groups on tumor tissues were analyzed by confocal microscopy and flow cytometry using Tz-Cy5 probe. Scale bar = 20 μm.