Fig. 4.

The artificial bioorthogonal targeting effectively boosted tumor clearance by CAR-T cells in blood cancer. (a) Raji-luci cells with or without BCN-motifs were intravenously inoculated into 6-week-old NOD/SCID mice. Six days later, mice were infused intravenously with indicated CAR-T cell every other week. (b) Tumor burden was monitored by bioluminescence imaging (BLI) every few days, and (c) quantified by the radiance detected in the region of interest (ROI). (d) The body weight change and (e) survival rate of mice in each group were separately monitored daily until day 40 post-inoculation. (f) On day 28, mouse organs, including heart, liver, spleen, lung, kidney, and thigh bone, were harvested and imaged by BLI, and (g) quantified by the radiance detected in the ROI. (h–i) The blood of mouse were harvest and the amount of peripheral Raji cells was measured by flow cytometry using anti-human CD19 antibodies. *p < 0.05.