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. 2020 Oct 14;11:5171. doi: 10.1038/s41467-020-18956-x

Fig. 6. Loss of IgSF8 impairs spontaneous synaptic transmission in CA3 neurons.

Fig. 6

a Representative sEPSC traces from whole-cell voltage-clamp recordings of CA3 neurons in acute hippocampal slices of Rbp4-Cre:Igsf8 cKO and WT littermates. b Cumulative distribution of sEPSC inter-event intervals. c Quantification of sEPSC frequency in littermate mice examined over three independent experiments (WT, n = 31 neurons and cKO, n = 38). **P = 0.008. d Cumulative distribution of sEPSC amplitudes. e Quantification of sEPSC amplitudes in littermate mice examined over three independent experiments (WT, n = 31 neurons and cKO, n = 38). **P = 0.002. f Cartoon illustrating whole-cell voltage-clamp recordings of CA3 neurons to measure MF-evoked responses in acute hippocampal slices of Rbp4-Cre:Igsf8 cKO and WT littermates using optogenetics. g Representative traces of paired-pulse ratio in Rbp4-Cre:Igsf8 cKO and WT littermates using optogenetics. h Quantification of first evoked amplitudes in littermate mice examined over three independent experiments (WT, n = 23 neurons and cKO, n = 21). i Quantification of paired-pulse ratios in littermate mice examined over three independent experiments (WT, n = 23 neurons and cKO, n = 21). Box-and-whisker plots in c and e show median, interquartile range, minimum, and maximum. Graphs in h and i show mean ± SEM. Two-sided Mann–Whitney tests were used in c and e. Source data are provided as a Source data file.