Skip to main content
. 2020 Oct 14;11:5171. doi: 10.1038/s41467-020-18956-x
Antibodies
Chicken anti-GFP Aves Labs

Cat#: GFP-1010;

RID: AB_2307313
Mouse anti-GFP clone B-2 Santa Cruz

Cat#: SC-9996;

RRID: AB_627695
Mouse anti-c-myc clone 9E10 Santa Cruz

Cat#: SC-40;

RRID: AB_627268
Rabbit anti-6x-His Tag Thermo-Fisher

Cat#: PA1-983B;

RRID: AB_1069891
Goat anti-MBP clone D-18 Santa Cruz

Cat#: SC-13912;

RRID: AB_648794
Mouse anti-PSD95 clone 7E3-1B8 Thermo-Fisher

Cat#: MA1-046;

RRID: AB_2092361
Rabbit anti-Histone H3 clone D1H2 Cell Signaling Technology

Cat#: 4499;

RRID: AB_10544537
Rabbit anti-Synaptophysin clone SVP-38 Sigma-Aldrich

Cat#: S5768;

RRID: AB_477523
Rat anti-Nectin3 clone 103-A1 Hycult Biotech

Cat#: HM1053;

RRID: AB_533278
Rabbit anti-Nectin3 Abcam

Cat#: AB63931;

RRID: AB_1142394
Mouse anti-GluK5/KA2 clone N279B/27 NeuroMab

Cat#: 75-362;

RRID: AB_2315855
Rabbit anti-Synaptoporin Synaptic Systems

Cat#: 102 002;

RRID: AB_887841
Guinea pig anti-VGluT1 Merck Millipore

Cat#: AB5905;

RRID: AB_2301751
Rabbit anti-Synapsin3 Synaptic Systems

Cat#: 106 303;

RRID: AB_2619775
Rabbit anti-Afadin clone A7L9H48 Thermo-Fisher

Cat#: 700193;

RRID: AB_2532299
Chicken anti-ZnT3 Synaptic Systems

Cat#: 197 006;

RRID: AB_2725754
Rabbit anti-mGluR2/3 Merck Millipore

Cat#: AB1553;

RRID: AB_11212089
Rabbit anti-CRTAC1 Merck Millipore Cat#: ABD80
Rabbit anti-BRINP2 Atlas Antibodies

Cat#: HPA061920;

RRID: AB_2684639
Goat anti-IgSF8 R&D Systems

Cat#: AF3117;

RRID: AB_2233385
Rabbit anti-FAM171A2 ThermoFisher

Cat#: PA5-71105;

RRID: AB_2689922
Rabbit anti-RPTPd Novus Cat#: NBP2-49153
Goat anti-RPTPs R&D Systems

Cat#: AF3430;

RRID: AB_2175157
Sheep anti-Teneurin-4 R&D Systems

Cat#: AF6320;

RRID: AB_10920937
Mouse anti-PSA-NCAM1 Merck Millipore

Cat#: MAB5324;

RRID: AB_95211
Rat anti-NCAM2 R&D Systems

Cat#: MAB778;

RRID: AB_2149709
Sheep anti-ISLR2 R&D Systems

Cat#: AF4650;

RRID: AB_2126610
Goat anti-NEGR1/Kilon R&D Systems

Cat#: AF5394;

RRID: AB_2150086
Mouse anti-Neuronal pentraxin 1 BD Biosciences

Cat#: 610369;

RRID: AB_397754
Goat anti-NRP1 R&D Systems

Cat#: AF566;

RRID: AB_355445
Goat anti-NRP2 R&D Systems

Cat#: AF2215;

RRID: AB_2155371
Sheep anti-Noelin R&D Systems

Cat#: AF4636;

RRID: AB_2157225
Goat anti-Plexin-A3 Thermo-Fisher

Cat#: PA5-47571;

RRID: AB_2608296
Rabbit anti-ROBO2 Aviva Systems

Cat#: ARP45396_P050;

RRID: AB_2047840
Goat anti-Neogenin R&D Systems

Cat#: AF1079;

RRID: AB_2151002
Sheep anti-SALM2 R&D Systems

Cat#: AF5669;

RRID: AB_1964706
Mouse anti-Tenascin-R R&D Systems

Cat#: MAB1624;

RRID: AB_2207001
Sheep anti-Contactin 1 R&D Systems Cat#: AF7549
Goat anti-CD200 R&D Systems Cat#: AF2724
Goat anti-ICAM5 Novus

Cat#: NB100-53815;

RRID: AB_829163
Goat anti-SCFR/Kit R&D Systems

Cat#: AF1356;

RRID: AB_354750
Mouse anti-LSAMP Developmental Studies Hybridoma Bank

Cat#: 2G9;

RRID: AB_2138210
Mouse anti-Trk-C R&D Systems

Cat#: MAB373;

RRID: AB_2155422
Goat anti-Plexin-A1 R&D systems

Cat#: AF4309;

RRID: AB_10645644
Rat anti-HA Roche

Cat#: 11867423001;

RRID: AB 390918
Rabbit anti-pERM Cell Signaling

Cat#: 3726;

RRID:AB 10560513
Goat anti-Chicken Alexa 488 Thermo-Fisher

Cat# A-11039;

RRID: AB_2534096
Donkey anti-Goat Alexa 488 Thermo-Fisher

Cat#: A-11055;

RRID: AB_2534102
Donkey anti-Goat Alexa 647 Thermo-Fisher

Cat#: A-21447;

RRID: AB_2535864
Donkey anti-Rabbit Alexa 488 Thermo-Fisher

Cat#: A-21206;

RRID: AB_2535792
Donkey anti-Rabbit Alexa 555 Thermo-Fisher

Cat#: A-31572;

RRID: AB_162543
Donkey anti-Rabbit Alexa 647 Thermo-Fisher

Cat#: A-31573;

RRID: AB_2536183
Donkey anti-Mouse Alexa 488 Thermo-Fisher

Cat#: A21202;

RRID: AB_141607
Donkey anti-Mouse Alexa 555 Thermo-Fisher

Cat#: A31570;

RRID: AB_2536180
Donkey anti-Mouse Alexa 647 Thermo-Fisher

Cat#: A-31571;

RRID: AB_162542
Donkey anti-Sheep Alexa 488 Thermo-Fisher

Cat# A-11015;

RRID: AB_2534082
Donkey anti-Sheep Alexa 647 Thermo-Fisher

Cat#: A-21448;

RRID: AB_2535865
Donkey anti-Chicken Alexa 488 Jackson ImmunoResearch

Cat# 703-545-155;

RRID: AB_2340375
Donkey anti-Rat Alexa 647 Jackson ImmunoResearch

Cat#: 712-605-153;

RRID: AB_2340694
Donkey anti-Guinea Pig Cy3 Jackson ImmunoResearch

Cat# 706-165-148;

RRID: AB_2340460
Donkey anti-Guinea Pig Alexa 647 Jackson ImmunoResearch

Cat#: 706-605-148;

RRID: AB_2340476
Donkey anti-Human IgG, Fc Fragment Cy3 Jackson ImmunoResearch

Cat#: 709-165-098;

RRID: AB_2340534
HRP Rabbit anti-Goat IgG Thermo-Fisher

Cat#: 81-1620;

RRID: AB_2534006
HRP Rabbit anti-Sheep IgG Thermo-Fisher

Cat#: 61-8620;

RRID: AB_2533942
HRP Goat anti-Mouse IgG Thermo-Fisher

Cat#: 62-6520;

RRID: AB_2533947
HRP Goat anti-Rabbit IgG Thermo-Fisher

Cat#: 65-6120;

RRID: AB_2533967
Placental alkaline phosphatase monoclonal antibody (8B6.18) Thermo-Fisher

Cat#: MA5-12694;

RRID: AB_10978663
HRP mouse anti-Human IgG1 Serotec Cat#: MCA514P
Bacterial and virus strains
AAV2/9-hsyn1-mGFP This paper N/A
AAV2/9-hsyn1-mGFP-T2A-Cre This paper N/A
AAV2/9-Ef1a-DIO-hChR2(E123T/T159C)-EYFP 126

Cat#: Addgene plasmid #35509;

RRID: Addgene_35509
Chemicals, peptides, and recombinant proteins
IgSF8-Fc This paper N/A
IgSF8-His Sino Biological Cat#: 51042-M08H
Tenascin-R-Fc This paper N/A
Brevican-Fc This paper N/A
Neurexin1β-Fc 127 N/A
Hoechst 33342 Thermo-Fisher Cat#: H3570
FM™ 4-64FX, fixable analog Thermo-Fisher Cat#: F34653
ChromPure Human IgG, Fc fragment Jackson Immunoresearch

Cat#: 009-000-008;

RRID: AB_2337046
AffiniPure Fab Fragment Donkey anti-Mouse IgG (H + L) Jackson Immunoresearch

Cat#: 715-007-003;

RRID: AB_2307338
FuGENE 6 Transfection Reagent Promega Cat#: E2691
AMAXA Nucleofector kit Lonza Cat#:VPG-1001
1-Step Ultra TMB-ELISA HRP Thermo-Fisher Cat#: 34028
Critical commercial assays
Myelin-Removal Beads II, human, mouse, rat Miltenyi Biotec Cat#: 130-096-733
Mix-n-Stain™ CF™ 488 A Antibody Labeling Kit Sigma-Aldrich

Cat#: MX488AS100;

RRID: AB_10961132
Pierce™ Antibody Clean-up Kit Thermo-Fisher Cat#: 44600
Gibson Assembly Cloning Kit New England Biolabs Cat#: E5510S
Deposited data
Proteomic analysis of sorted MF synaptosomes and P2 synaptosomes Proteome Exchange PXD013492
Experimental models: cell lines
Human: HEK293T cells ATCC

Cat#: CRL-11268

RRID: CVCL_1926
Human: HEK293F suspension adapted Thermo-Fisher Cat # R790-07
Mouse: hippocampal primary neurons cultured from embryonic day 18 Igsf8 conditional knock out mice This paper N/A
Experimental models: organisms/strains
Mouse: Igsf8 cKO (B6D2;129S2-Igsf8tm1.1Osb) Riken Institute64

Cat#: RBRC05637;

RRID: IMSR_RBRC05637
Mouse: Tg(Rbp4-cre)KL100Gsat GENSAT

Cat#: 031125-UCD;

RRID: MMRRC_031125-UCD
Mouse: C57BL/6 J JAX

Cat#: 000664;

RRID: IMSR_JAX:000664
Recombinant DNA
Plasmid: FUGW-mCherry 115 N/A
Plasmid: FUGW-CRE-T2A-mCherry 115 N/A
Plasmid: FUGW-mGFP-T2A-HA-IgSF8 This paper N/A
Plasmid: FUGW-GFP-T2A-HA-IgSF8 This paper N/A
Plasmid: pAAV-hsyn1-mGFP This paper N/A
Plasmid: pAAV-hsyn1-mGFP-T2A-Cre This paper N/A
Plasmid: pAAV-Ef1a-DIO-hChR2(E123T/T159C)-EYFP 126 Cat#: Addgene plasmid #35509; RRID:Addgene_35509
Plasmid: pBOS-EGFP 128 N/A
Plasmid: pBOS-myc-LRRTM2 127 N/A
Plasmid: pEGFP-N1 Clontech (TaKaRa) N/A
Plasmid: pEGFP-N1-IgSF8 This paper N/A
Plasmid: pEGFP-N1-Tenascin-R This paper N/A
Plasmid: 4F2hc-Fc/AP This paper
Plasmid: ADAM11-Fc/AP This paper
Plasmid: ADAM22-Fc/AP This paper
Plasmid: ADAM23-Fc/AP This paper
Plasmid: ALCAM-Fc/AP This paper
Plasmid: APMAP-Fc/AP This paper
Plasmid: BRINP2-Fc/AP This paper
Plasmid: CADM4-Fc/AP This paper
Plasmid: CD200-Fc/AP This paper
Plasmid: CNTN1-Fc/AP This paper
Plasmid: CNTNAP2-Fc/AP This paper
Plasmid: CRTAC1-Fc/AP This paper
Plasmid: ELFN1-Fc/AP This paper
Plasmid: ELFN2-Fc/AP This paper
Plasmid: EPHA4-Fc/AP This paper
Plasmid: EPHA6-Fc/AP This paper
Plasmid: EPHA7-Fc/AP This paper
Plasmid: EPHB2-Fc/AP This paper
Plasmid: EPHB4-Fc/AP This paper
Plasmid: FAM171A2-Fc/AP This paper
Plasmid: GPR37L1-Fc/AP This paper
Plasmid: HAPLN1-Fc/AP This paper
Plasmid: HEPACAM-Fc/AP This paper
Plasmid: ICAM5-Fc/AP This paper
Plasmid: IgSF8-Fc/AP This paper
Plasmid: ISLR2-Fc/AP This paper
Plasmid: KIT/SCFR-Fc/AP This paper
Plasmid: L1CAM-Fc/AP This paper
Plasmid: LGI1-Fc/AP This paper
Plasmid: LINGO1-Fc/AP This paper
Plasmid: Lphn1-Fc/AP This paper
Plasmid: Lphn3-Fc/AP This paper
Plasmid: LRFN1/SALM2-Fc/AP This paper
Plasmid: LRRC4B/NGL3-Fc/AP This paper
Plasmid: LSAMP/IgLON3-Fc/AP This paper
Plasmid: NCAM1-Fc/AP This paper
Plasmid: NCAM2-Fc/AP This paper
Plasmid: Nectin3-Fc/AP This paper
Plasmid: NEGR1/IgLON4-Fc/AP This paper
Plasmid: Neogenin-Fc/AP This paper
Plasmid: Neurofascin-Fc/AP This paper
Plasmid: NLGN1-Fc/AP This paper
Plasmid: NLGN2-Fc/AP This paper
Plasmid: NLGN3-Fc/AP This paper
Plasmid: NPTN-Fc/AP This paper
Plasmid: NPTX1-Fc/AP This paper
Plasmid: Noelin-Fc/AP This paper
Plasmid: NrCAM-Fc/AP This paper
Plasmid: NRP1-Fc/AP This paper
Plasmid: NRP2-Fc/AP This paper
Plasmid: NRXN1α-Fc/AP This paper
Plasmid: NRXN3α-Fc/AP This paper
Plasmid: NRXN1β-Fc/AP This paper
Plasmid: NRXN3β-Fc/AP This paper
Plasmid: Neurotrimin/IgLON2-Fc/AP This paper
Plasmid: PlexinA1-Fc/AP This paper
Plasmid: PlexinA2-Fc/AP This paper
Plasmid: PlexinA3-Fc/AP This paper
Plasmid: PlexinA4-Fc/AP This paper
Plasmid: PlexinB1-Fc/AP This paper
Plasmid: PlexinC1-Fc/AP This paper
Plasmid: RPTPα-Fc/AP This paper
Plasmid: RPTPδ-Fc/AP This paper
Plasmid: RPTPf/LAR-Fc/AP This paper
Plasmid: RPTPσ-Fc/AP This paper
Plasmid: ROBO1-Fc/AP This paper
Plasmid: ROBO2-Fc/AP This paper
Plasmid: Shisa6-Fc/AP This paper
Plasmid: SIRPα-Fc/AP This paper
Plasmid: Teneurin4-Fc/AP This paper
Plasmid: TenR-Fc/AP This paper
Plasmid: TrkC-Fc/AP This paper
Plasmid: VCAM1-Fc/AP This paper
Mouse IgSF8 cDNA clone Origene MC206708
Mouse Tenascin-R cDNA clone Source Bioscience IRCKp5014L0710Q
Mouse Brevican cDNA clone Source Bioscience IRAVp968B01111D
Software and algorithms
Fiji NIH

https://imagej.net/Welcome;

RRID: SCR_002285
Imaris BITPLANE

http://www.bitplane.com/imaris;

RRID: SCR_007370
Microscope Image Browser (MIB) University of Helsinki http://mib.helsinki.fi/index.htm
GraphPad Prism8 GraphPad Software, Inc

https://www.graphpad.com/scientific-software/prism/;

RRID: SCR_002798