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. 2020 Oct 14;11:5155. doi: 10.1038/s41467-020-18958-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Design of the cold-shock-triggered temperature control system based on Gal4M9 and wild-type Gal4. After deletion of GAL80, the P_GAL4-driven Gal4M9 is expressed upon glucose depletion, and its activity is controlled by temperature. When the temperature is changed from 30 to 24 °C, the activated Gal4M9 switches on the expression of the wild-type Gal4 under control of the weakened P_GAL1-3, and the accumulated Gal4 activates the expression of P_GAL-driven genes including its own encoding gene. Even when the temperature is switched back to 30 °C after cold shock, the pathway can be kept activated in the presence of sufficient Gal4. b Biomass and tocotrienols yield of YS-M5 under regulation of different temperature control systems in shake-flask cultures. All values presented are the means of three biological replicates, and error bars represent standard deviations. Source data are provided as a Source Data file.