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. 2020 Oct 14;10:17240. doi: 10.1038/s41598-020-73974-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Estrogen receptors expression from whole kidney and uterus weight at day 1 (a to d) and day 21 (e to h) after hemorrhagic shock. (a and e) estrogen impregnation assessed by measure of uterus weight; (b,f) expression of GPR30; (c,g) expression of estrogen-receptor α; (d,h) expression of estrogen-receptor β. Sham: non-shocked mice; Ov: ovariectomized mice; Ov + cE2: ovariectomized mice with chronic estradiol substitution; Intact + V: Non ovariectomized mice receiving vehicle; Intact + aE2: intact mice receiving 25 µg of estradiol at the end of resuscitation. N = 6 in each group. Relative mRNA expression levels were calculated using the ΔΔCtCt method, normalized to HPRT and β actin, and expressed as fold changes relative to the sham animals. Data are presented as mean ± s.e.m. *P < 0.05 versus sham group; ^##P < 0.001 and ^###P < 0.001 for indicated comparison.