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. 2020 Oct 14;10:17240. doi: 10.1038/s41598-020-73974-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell death at day 1. (a) picture of renal cortex stained by TUNEL method (magnification X800) and quantification of stained nuclei, n = 6 for all groups. Apoptosis at day 1 (b to d): (b) Caspase 8 mRNA expression (from whole kidney); (c) Western Blot of the cleaved-form of caspase 8; (d) quantification of the expression of cleaved-form of caspase 8 from 4 independent Western-Blot. Sham: non-shocked mice; Ov: ovariectomized mice; Ov + cE2: ovariectomized mice with chronic estradiol substitution; Intact + V: non ovariectomized mice receiving vehicle; Intact + aE2: intact mice receiving 25 µg of estradiol at the end of resuscitation. Relative mRNA expression levels were calculated using the ΔΔCtCt method, normalized to HPRT and β actin, and expressed as fold changes relative to the sham animals. Data are presented as mean ± s.e.m. *P < 0.05 versus sham; ^#P < 0.05 and ^##P < 0.01 for indicated comparison.