Table 1.
CHIKV-181/25-derived LAVs are not efficiently transmitted by Ae. aegypti.
| Virus | Bloodmeal titer (PFU/mL) | Infected (infectious CHIKV) | Transmitting (infectious CHIKV) | Infected (CHIKV RNA) | Transmitting (CHIKV RNA) |
|---|---|---|---|---|---|
| CHIKV-181/25 | 6.5 × 106 | 23% (7/30) | 0% (0/30) | 37% (11/30) | 0% (0/30) |
| CHIKV-181/25-P2.P4 | 1.6 × 107 | 30% (9/30) | 3% (1/30) | 37% (11/30) | 3% (1/30) |
| CHIKV-181/25-P2.P4.E3/E1 | 1.8 × 105 | 10% (3/30) | 0% (0/30) | 20% (6/30) | 0% (0/30) |
| CHIKV-06-049 | 4.5 × 107 | 30% (7/23) | 9% (2/23) | 44% (10/23) | 22%C (5/23) |
Ae. aegypti mosquitoes colonized from Los Angeles, California, USA, were presented bloodmeals containing LAVs at titers reported. Saliva was collected to assess transmission rates and whole bodies were assayed for infection rate assessments 10 days post blood feeding. Infectious CHIKV and RNA were determined by plaque assay and qRT-PCR, respectively. Table values are percentage (positive/total). P < 0.001 (C) Chi-square test, n = 23–30, 1 experiment.