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. 2020 Oct 14;10:17185. doi: 10.1038/s41598-020-74092-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Response of mixed microbes treated with Eucalyptus extract –PSM and optimization of E-dose (a) Tannin-protein complex formation after 24 h with increasing order of Eucalyptus-dose (indicated by red arrow). (b) Effect of increasing order of Eucalyptus extract-dose on the viability of mixed microbiota represented by plate count (CFC per ml) after 24 h of incubation at 30 ˚C. (c) Represent different morphological growth of microbes, wrinkle and ring structures on petri-plates with increasing order of Eucalyptus-dose. (d) The pale yellow colour pictures are Gram stained microbes and the red colour pictures are nucleic acid stained by acryl orange. The change in cell morphology, filamentous growth and spheroplast formation are displayed with respect to different concentrations of Eucalyptus-doses (0.5 mg, 1.0 mg, 1.5 mg, 2.0 mg and 5.0 mg) per 1 ml of sludge with VSS of 25 g.l^-1. (e) Gram staining of mixed microbes (for cell wall). (f) Acrylic orange staining for nucleic acid. Graphs are plotted by using Origin 9.0 software (https://www.originlab.com/).