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. 2020 Oct 14;10:17269. doi: 10.1038/s41598-020-74042-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NFκB activity is not affected by wtHTT lowering in human macrophages. Primary macrophages treated with either anti-HTT or scrambled siRNA-containing GeRPs and stimulated with 2 µg/ml LPS and 10 ng/ml IFNγ for 45 min, were analysed by imaging flow cytometry for RELA translocation into the cell nucleus. RELA is one subunit of the heterodimeric complex that comprises the classical/canonical form of NFκB. (A) Example images are shown of cells in which RELA had or had not translocated into the nucleus as determined by overlapping RELA (yellow) and Hoechst (purple). (B) There was no significant difference in the percentage of total cells showing RELA translocation into the nucleus (n = 4). Data are presented as mean ± SEM, analysed by paired two-tailed Student’s t-test.