Figure 5.

Wild-type HTT lowering increases phagocytosis by human macrophages and their susceptibility to stress. (A) Phagocytic activity, assessed using either zymosan or E. coli pHrodo beads, was significantly increased in primary human macrophages treated with anti-HTT siRNA-GeRPs compared to those treated with scrambled siRNA (n = 4–5; *p < 0.05, **p < 0.01). (B) Viability as assessed by LDH release of primary human macrophages treated with anti-HTT siRNA-GeRPs was not altered compared to those treated with scrambled siRNA (n = 6), unless the cells were exposed to a stress, bafilomycin A1, for nine hours (n = 3; **p < 0.01). Data are presented as mean ± SEM, analysed by paired two-tailed Student’s t-test.