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. 2020 Oct 14;10:17221. doi: 10.1038/s41598-020-73891-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Downregulation of M1BP suppresses the eye fate during Drosophila eye development. (A) ey-Gal4 driven expression of UAS-GFP transgene in the eye. Note that ey > GFP (green) expressed in the entire eye field of third instar larval eye disc. Note that eye imaginal disc is stained with pan neuronal marker Elav (red) which marks the nuclei of retinal neurons and Dlg, a membrane specific marker, to mark outline of the tissue. (B) Adult eye. (C–F) Downregulation of M1BP in the eye by driving expression of UAS-M1BP^RNAi (ey > M1BP^RNAi) suppresses the eye fate as seen in (C,E) the eye imaginal disc and the (D,F) adult eye. ey > M1BP^RNAi exhibits a range of eye suppression phenotype ranging from a (C,D) small-eye to a "no-eye". (G) The area of adult eye was quantified using Image J software (NIH). The p values for the eye size (μm²) were calculated in a set of five (n = 5) using Student’s t-test in MS Excel Software. ey-Gal4 was found to be statistically significant from ey > M1BP^RNAi in case of both small-eye (p < 0.001, ***) and no-eye phenotype (p < 0.001, ***). The orientation of all imaginal discs is identical with posterior to the left and dorsal up. The magnification of all eye-antennal imaginal disc is 20 × and the adult eye is 10 ×. A total of five eye-antennal imaginal discs (n = 5) for each genotype were analyzed for respective immunohistochemistry staining.