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. 2020 Oct 14;11(10):858. doi: 10.1038/s41419-020-03047-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Hierarchically clustered heatmap of differentially expressed genes in PC9/GR cells after transfection of CASC9 or control siRNAs. b Nine representative genes mRNA levels in PC9/GR cells depleted of CASC9. c Western blot analysis of DUSP1 expression in PC9/GR cells transfected with CASC9 siRNA and CASC9-ASO. d qRT-PCR analysis was performed to determine the subcellular localization of CASC9 in PC9/GR cells. e RIP assays were performed in PC9/GR cells to show CASC9 co-immunoprecipitation with EZH2, LSD1, SUZ12 and Ago2. f qRT-PCR analysis of EZH2 expression in PC9 and PC9/GR cells. g IC50 values of gefitinib in EZH2-depleted PC9/GR cells was examined by CCK8 assay. h Colony formation assays were used to evaluate the colony formation capacity of PC9/GR cells depleted of EZH2 treated with gefitinib. i FACS analysis of the effect of EZH2 down-regulation on PC9/GR cells apoptosis treated with gefitinib. j, k qRT-PCR and western blot analysis of EZH2 and DUSP1 mRNA and proteins expression in PC9/GR cells transfected with siRNA-NC or si-EZH2. l ChIP-qPCR assay showing EZH2 occupancy on the DUSP1 promoters was reduced by CASC9 knockdown. *P < 0.05, **P < 0.01.