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. 2020 Oct 14;11(10):858. doi: 10.1038/s41419-020-03047-y

Fig. 5. Upregulation of DUSP1 increases the sensitivity of PC9/GR cells to gefitinib and regulates ERK pathway.

Fig. 5

a qRT-PCR analysis of DUSP1 expression in PC9 and PC9/GR cells. b qRT-PCR analysis of DUSP1 mRNA levels in PC9/GR cells overexpressing DUSP1. c CCK8 assays were performed to determine the IC50 ability of DUSP1-overexpressing PC9/GR cells. d, e CCK8 and colony formation assays were performed to determine the proliferation of DUSP1-overexpressing PC9/GR cells treated with gefitinib. f FACS analysis of PC9/GR cells apoptosis overexpressing DUSP1 treated with gefitinib. g Western blot analysis of ERK and p-ERK proteins expression in PC9/GR cells transfected with DUSP1 vector. *P < 0.05, **P < 0.01.