Figure 2.

(a) Schematic illustration of RFLP. The BtgI restriction enzyme can cut the BFP but not wild type of GFP or restored GFP (C to U converted) (b) PCR–RFLP of cDNA extracted from transfected cells (HEK 293 stably expressing BFP), restriction-digested with BtgI. BFP was cleaved into two fragments, 201 and 123 bp, whereas restored GFP was not cleaved and remained at 324 bp. (c) For all data the statistical analysis (mean ± SEM) was calculated, where n = 5, Image J software was used for measuring the band intensity (https://imagej.net/Citin).