Table 2.

Comparison of inter-sample gene expression in the prospective and retrospective cohorts. Mean (Ct), inter-sample SD and inter-sample CoV in gene expression (Ct) in FFPE tissue from prospective (12 samples) and retrospective (16 samples) bladder cancer cohorts.

Gene	Prospective samples (n = 12)			Retrospective samples (n = 16)			P value*
	Mean Ct	SD	CoV (%)	Mean Ct	SD	CoV (%)
TaqMan™ endogenous control array
`18S rRNA	23.29	1.71	7.60	30.43	2.39	7.86	4.60E−07
ACTB	22.09	1.26	5.71	29.50	4.85	16.47	9.20E−06
B2M	16.27	0.75	4.62	20.11	1.56	7.80	6.57E−08
GAPDH	18.51	1.19	6.44	23.44	2.18	9.29	7.53E−07
GUSB	20.15	0.86	4.25	24.06	1.56	6.49	2.63E−07
HMBS	23.08	0.96	4.18	25.58	1.21	4.71	2.95E−06
HPRT1	22.35	1.23	5.49	29.61	5.36	18.09	9.17E−07
IPO8	20.48	0.71	3.45	23.87	1.12	4.70	6.57E−08
PGK1	17.31	0.82	4.75	20.80	1.44	6.95	7.89E−07
POLR2A	19.37	0.70	3.59	21.99	0.80	3.64	6.57E−08
PPIA	16.71	1.10	6.59	20.38	1.59	7.78	2.63E−07
RPLP0	18.15	1.10	6.02	22.59	1.94	8.60	1.31E−07
TBP	25.57	1.13	4.40	31.92	4.16	13.03	9.17E−06
TFRC	20.40	1.20	5.88	23.85	1.50	6.28	7.89E−07
UBC	16.94	0.88	5.24	20.35	1.18	5.80	6.57E−08
YWHAZ	21.90	0.98	4.49	24.99	1.57	6.29	2.63E−07
Single tube assay
SDHA (70)	20.18	0.81	4.00	23.00	1.37	6.00	1.28E−05
RPL11 (142)	18.90	1.17	6.17	24.53	2.50	10.2	2.63E−07
RPL11 (106)	17.40	1.06	6.09	21.91	1.91	8.70	2.63E−07
RPL24 (156)	19.00	1.19	6.30	24.93	2.43	9.75	1.32E−07
RPL24 (86)	16.69	1.02	5.78	20.26	1.52	7.47	2.63E−07
GNB2L1 (75)	17.17	0.99	5.24	21.50	1.69	7.86	6.57E−08
GNB2L1 (66)	16.35	0.86	6.11	19.23	1.15	5.98	2.63E−07

*The p values are from a Mann Whitney test comparison of mean Ct values in the prospective and retrospective data.