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. 2020 Oct 14;10:17248. doi: 10.1038/s41598-020-74192-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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There are no significant differences in age matched CSA and myofiber number between mdx and WT. However, there is greater variability in the CSA of mdx muscles relative to WT. (A–C) CSA of adult and aged mdx and WT EDL, SOL and STM muscles. There are no significant differences between groups. (D) Myofiber numbers in WT and mdx EDL muscles. There are no differences in myofiber number between mdx and WT age matched groups; however, both WT and mdx EDL muscles lose a significant number of myofibers as they age. (E) Myofiber numbers in WT and mdx SOL muscles. There are no differences in myofiber number between any groups in the SOL muscle. (F) Myofiber numbers in WT and mdx STM muscles. There are no differences in myofiber number between any groups in the STM muscle. Analysis via two-way ANOVA with Tukey post hoc test. * = p < 0.05. All data sets are displayed as mean ± s.e.m.