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. 2020 Oct 14;11(10):856. doi: 10.1038/s41419-020-03073-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A 7 DIV hippocampal neurons were infected with either mTurquoise-tagged Scramble or ShPRRT2 or co-infected with mTurquoise-ShPRRT2 and rPRRT2-mCherry lentiviral vectors. After 7 days (14 DIV), rods were stained using cofilin antibody and recognized as dense elongated bundles along neurites as shown in the insets. Scale bar: 20 μm (Insets: 25 μm). B Quantitative analysis of the rod density expressed as number of rods per field. Mean ± SEM n. rods/field: Scramble = 0.170 ± 0.05, ShPRRT2 = 0.641 ± 0.09, Sh+rPRRT2 = 0.477 ± 0.12. Data are expressed as mean ± SEM of the number of rods found in each experiment from five independent experiments. For each coverslip at least 20 random fields were acquired. One-way ANOVA/Bonferroni’s tests; *p < 0.05.