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. 2020 Sep 26;88(8):662–673. doi: 10.1007/s00239-020-09965-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Schematic phylogenetic tree from MUSCLE alignment. The full tree is in Supplementary Figure S1. aLRT-SH over 0.5 are shown, with branches below 0.5 being collapsed. We have not attempted to precisely define the different clades, but have indicated the locations of the main subfamilies with reference to Fig. 1 and Mei and Dvornyk (2015). N1–N3 refer to the three nodes discussed in the manuscript. The Figure highlights the positions of animal sequences (in black) relative to α-proteobacterial sequences (in magenta) and cyanobacterial sequences (in cyan). Also colour coded are sequences from other bacteria (blue), archaea (orange), fungi (red), diatoms (brown), green algae (light green) and plants (dark green) (Color figure online)