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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Int J Cancer. 2012 Sep 28;132(8):1771–1780. doi: 10.1002/ijc.27798

Figure 2.

Figure 2.

Promoter hypermethylation of SFRP genes in prostate cancer. MSP and pyrosequencing of prostate cancer cell lines and tumors. Representative CpG islands are shown for SFRP1, SFRP2, SFRP3, SFRP4 and SFRP5. Individual vertical lines indicate the presence of a CpG dinucleotide and the location of sequenced CpG sites is indicated by a horizontal line. The transcriptional start site is shown by an arrow, and the first coding exon is illustrated by a thick black box. Each square represents the amount of methylation at a given CpG site, shaded from white (unmethylated) to black (fully methylated). Each row depicts the sequencing pattern for an individual sample, summarized in the bar charts. Dashed lines in the bar charts represent the mean level of methylation detected in an unmethylated sample. Abbreviations: PC-3 cells treated with 5AzaCdR are shown by PC-3+T, LNCaP: LN, DU145: DU, 22Rv1: 22R, PWR1E: PW, RWPE1: RW, MC: universal human methylated DNA, UCA and UCB: universal unmethylated DNA from human genomic DNA and a human fetal cell line, respectively.