Figure 3.

Hyperpolarized ¹³C metabolic images of a TRAMP mouse. Upper: Hyperpolarized ¹³C lactate image following the injection of hyperpolarized [1-¹³C]pyruvate, overlaid on T₂-weighted ¹H image. Middle: Hyperpolarized ¹³C spectra of primary and metastatic tumor regions. Lower: Representative H&E-stained sections and hyperpolarized ¹³C spectra for one case from each of the histologically defined groups. The 3D MRSI shows substantially elevated lactate in the high-grade primary tumor compared with the low-grade tumor. Ala, alanine; Lac, lactate; Pyr, pyruvate (see [22]).