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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Biochim Biophys Acta Bioenerg. 2020 Aug 20;1861(12):148290. doi: 10.1016/j.bbabio.2020.148290

Fig. 1.

Fig. 1.

Time-dependent changes in rhodamine 123 fluorescence (excitation λ503 nm; emission λ527 nm), as an indicator of membrane potential (ΔΨm) in guinea pig isolated mitochondria undergoing stress induced by: CaCl2 (75 μM) added at 90 s and Na-succinate (10 mM) added at 140 s (CaCl2 + succinate); Na-Succinate (10 mM) added first at 90 s and CaCl2 (75 μM) added at 150 s (succinate + CaCl2 ) did not depolarize ΔΨm. Pretreatments of vehicle, rotenone (10 μM), antimycin A (5 μM) and rotenone + antimycin A were added to the buffer before adding mitochondria and exposure to CaCl2 and succinate. Tracings shown were similar in mitochondria isolated from two other guinea pig hearts.