Fig. 2. TRPM7 current amplitudes during Mg²⁺ loading and depletion. Time course of Mg²⁺ depletion.

Dependence of TRPM7 preactivation index (a) and maximum amplitude (b) on the duration of Mg²⁺ depletion (up to 60 hrs). c. Maximum TRPM7 current amplitudes in Jurkat T cells incubated in 1.4 mM and 8 μM [Mg²⁺] for 1 day. 3 cells prior to 1.4 Mg²⁺ were exposed to 8 μM Mg²⁺. In a–c, Chelex-RPMI was supplemented with 0.42 mM [CaCl₂]. TRPM7 current amplitudes were measured at 83.77 mV (a, b) and 83.42 mV (c). d. RT-PCR of total RNA isolated from Jurkat T cells incubated in 8 μM and 0.4 mM Mg²⁺ in the presence of 0.42 mM CaCl₂ for 1 day. Primers for TRPM7 and GAPDH were used with predicted amplicon sizes of 734 and 555 bp, respectively. In lanes marked –RT here and in Fig. 4a, reverse transcriptase was omitted from the reaction.