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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Process Biochem. 2020 Sep 25;100:39–48. doi: 10.1016/j.procbio.2020.09.029

Figure 2. Detection of recombinant SCF expression by an anti-SCF Western blot.

Figure 2.

(a) SCF (native gene sequence) expressed in E. coli and tobacco plants (transient expression in N. benthamiana). pET28b(+) expression vector with a T7 promoter was used for the E. coli expression as previously described [14]; (b) Detection of the glycosylation of the SCF expressed in N. benthamiana. SCF crude extract was incubated with (+) and without (−) PNGase F. (New England Biolab, MA) at 37°C for 1 hour before the assay by Western blot; (c) Comparison of the SCF expression in N. benthamiana between using the native gene sequence (nat) and the codon optimized SCF gene sequence (opt). The tobacco leaves collected 48 hr and 72 hr post-infiltration were analyzed. WT: wild-type tobacco; std: SCF standard (30 ng).