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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Process Biochem. 2020 Sep 25;100:39–48. doi: 10.1016/j.procbio.2020.09.029

Figure 3. Anti-SCF Western blot detection of the recombinant SCF, (SP)20-SCF and SCF-(SP)20 expressed in BY-2 cells.

Figure 3.

(a) Secreted (SP)20-tagged SCF in BY-2 cell cultures for 10 days. Secreted SCF was undetectable. M: molecular marker; std: SCF standard (30 ng); (b) Intracellular SCF and (SP)20-tagged SCF in cultured BY-2 cells. Both the glycosylated (~21 kDa) and non-glycosylated form (18.5 kDa) of SCF was detected. The (SP)20 tag substantially increases the apparent molecular size of SCF to 35 kDa; (c) and (d) Time course of the (SP)20-SCF and SCF-(SP)20 secretion in BY-2 cell cultures, respectively