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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Process Biochem. 2020 Sep 25;100:39–48. doi: 10.1016/j.procbio.2020.09.029

Figure 4. Purification of the (SP)20-tagged SCF from BY-2 cell culture media.

Figure 4.

(a) Anti-SCF Western blot assay of the SCF-(SP)20 fractions collected during the nickel affinity chromatography. FT: flow through; 3xW: three times wash with a 2-column volume of equilibration buffer each time; E1-E5: five times elution with a 2-column volume of elution buffer each time; HIC: protein solution eluted from HIC column; (b) SDS-PAGE separation of the purified (SP)20-tagged SCF. The protein bands were detected by the Coomassie brilliant blue staining and silver staining, respectively. M: molecular maker; C: SCF with a C-terminal tag or SCF-(SP)20; N: SCF with a N-terminus tag or (SP)20-SCF