Figure 6.

gemR PDX models retained mutations in codon 12 of the KRAS gene that were present in parent PDX models. Human-specific primers amplified the expected 214 base pair PCR product that included codons 12 and 13 of exon 2 of the KRAS gene. DNA from mouse tissue was used as a negative control (A); electropherograms document retention by gemR PDX models of mutations in codon 12 of the KRAS gene that are present in respective parent models (B). gemR: gemcitabine resistance; PDX: patient-derived xenograft