Table 2. Overview of samples used in the experiment.
When no data is available, “nd” is reported. For the Scanning Electron Microscopy (SEM) imaging “None” is reported when the SEM analysis yielded no detectable tunneling, when Wedl attack is observed, “Wed” is reported in the table, while “bac” relates to non-Wedl microbial tunneling.
| ID | Pretreatment | Environment | Exposure time (weeks) | SEM | #Reads before filtering | #Reads after filtering | #unfiltered ZOTUs |
|---|---|---|---|---|---|---|---|
| 20 | raw | Control | 0 | None | 10709 | 7437 | 83 |
| 9 | raw | Terrestrial sand | 4 | nd | 2425 | 2298 | 95 |
| 10 | raw | Terrestrial sand | 14 | nd | 2120 | 2079 | 227 |
| 11 | raw | Terrestrial sand | 28 | nd | 87947 | 87316 | 1190 |
| 12 | raw | Terrestrial sand | 52 | None | 49614 | 49405 | 1420 |
| 6 | raw | Tidal zone | 14 | nd | 208092 | 207852 | 2614 |
| 7 | raw | Tidal zone | 28 | nd | 50839 | 50707 | 1205 |
| 8 | raw | Tidal zone | 52 | Wed | 195181 | 193579 | 3982 |
| 1 | raw | Submerged gyttja | 4 | nd | 241228 | 217896 | 476 |
| 2 | raw | Submerged gyttja | 14 | nd | 156777 | 152735 | 972 |
| 3 | raw | Submerged gyttja | 28 | nd | 64313 | 64127 | 562 |
| 4 | raw | Submerged gyttja | 52 | None | 129353 | 127610 | 1549 |
| 13 | raw | Submerged sand (spring) | 4 | nd | 84195 | 83856 | 398 |
| 14 | raw | Submerged sand (spring) | 14 | nd | 1126173 | 1052704 | 1477 |
| 15 | raw | Submerged sand (spring) | 28 | nd | 56953 | 52337 | 1443 |
| 17 | raw | Submerged sand (autumn) | 4 | nd | 111144 | 107584 | 894 |
| 18 | raw | Submerged sand (autumn) | 14 | nd | 120732 | 115678 | 751 |
| 19 | raw | Submerged sand (autumn) | 28 | nd | 93207 | 86430 | 503 |
| 21 | Boiled | Control | 0 | None | 14759 | 1336 | 45 |
| 30 | Boiled | Terrestrial sand | 4 | nd | 137500 | 137262 | 277 |
| 31 | Boiled | Terrestrial sand | 14 | nd | 117750 | 117052 | 1233 |
| 32 | Boiled | Terrestrial sand | 28 | nd | 38183 | 38131 | 1049 |
| 33 | Boiled | Terrestrial sand | 52 | bac | 819814 | 817121 | 3736 |
| 26 | Boiled | Tidal zone | 4 | nd | 33757 | 33725 | 1275 |
| 27 | Boiled | Tidal zone | 14 | nd | 32493 | 32462 | 1977 |
| 28 | Boiled | Tidal zone | 28 | nd | 49857 | 49833 | 1680 |
| 29 | Boiled | Tidal zone | 52 | Wed | 100833 | 96043 | 3155 |
| 22 | Boiled | Submerged gyttja | 4 | nd | 138580 | 137285 | 469 |
| 23 | Boiled | Submerged gyttja | 14 | nd | 98111 | 86902 | 875 |
| 24 | Boiled | Submerged gyttja | 28 | nd | 45450 | 42632 | 814 |
| 25 | Boiled | Submerged gyttja | 52 | None | 36423 | 35101 | 1105 |
| 34 | Boiled | Submerged sand (spring) | 4 | nd | 237401 | 236387 | 717 |
| 35 | Boiled | Submerged sand (spring) | 14 | nd | 98517 | 97975 | 722 |
| 36 | Boiled | Submerged sand (spring) | 38 | None | 87294 | 86451 | 1435 |
| 38 | Boiled | Submerged sand (autumn) | 4 | nd | 196378 | 188020 | 522 |
| 39 | Boiled | Submerged sand (autumn) | 14 | nd | 180136 | 169312 | 883 |
| 40 | Boiled | Submerged sand (autumn) | 28 | nd | 121835 | 99959 | 475 |
| 41 | Baked | Control | 0 | None | 3658 | 3297 | 65 |
| 50 | Baked | Terrestrial sand | 4 | nd | 249321 | 235708 | 377 |
| 51 | Baked | Terrestrial sand | 14 | nd | 245545 | 245482 | 857 |
| 52 | Baked | Terrestrial sand | 28 | nd | 107735 | 106871 | 1994 |
| 53 | Baked | Terrestrial sand | 52 | None | 151935 | 151260 | 3337 |
| 46 | Baked | Tidal zone | 4 | nd | 57872 | 57756 | 1320 |
| 47 | Baked | Tidal zone | 14 | nd | 93216 | 93202 | 719 |
| 48 | Baked | Tidal zone | 28 | nd | 97871 | 97794 | 2279 |
| 49 | Baked | Tidal zone | 52 | Wed | 562700 | 561684 | 6910 |
| 42 | Baked | Submerged gyttja | 4 | nd | 82808 | 82462 | 436 |
| 43 | Baked | Submerged gyttja | 14 | nd | 57087 | 55962 | 1027 |
| 44 | Baked | Submerged gyttja | 28 | nd | 100756 | 90923 | 716 |
| 45 | Baked | Submerged gyttja | 52 | Wed | 42336 | 41668 | 790 |
| 54 | Baked | Submerged sand (spring) | 4 | nd | 271449 | 270124 | 454 |
| 55 | Baked | Submerged sand (spring) | 14 | nd | 110663 | 108808 | 1632 |
| 56 | Baked | Submerged sand (spring) | 38 | None | 170111 | 159595 | 1261 |
| 58 | Baked | Submerged sand (autumn) | 4 | nd | 149706 | 145103 | 487 |
| 59 | Baked | Submerged sand (autumn) | 14 | nd | 127646 | 125271 | 840 |
| 60 | Baked | Submerged sand (autumn) | 28 | nd | 62732 | 59420 | 850 |
| 82 | Sediment | Terrestrial sand | 0 | nd | 552332 | 540883 | 5774 |
| 93 | Sediment | Terrestrial sand | 52 | nd | 687780 | 674948 | 6019 |
| 80 | Sediment | Tidal zone | 0 | nd | 954600 | 945656 | 8800 |
| 83 | Sediment | Tidal zone | 4 | nd | 1155354 | 1150669 | 6232 |
| 85 | Sediment | Tidal zone | 52 | nd | 32169 | 32077 | 3022 |
| 81 | Sediment | Submerged sand | 0 | nd | 1035338 | 1026415 | 10565 |
| 86 | Sediment | Submerged sand | 14 | nd | 33911 | 33459 | 2995 |
| 88 | Sediment | Submerged sand | 38 | nd | 827015 | 811393 | 9742 |
| 61 | nd | Extraction blank | nd | nd | 341 | 341 | 30 |
| 62 | nd | Extraction blank | nd | nd | 19 | 19 | 4 |
| N5 | nd | Extraction blank | nd | nd | 18 | 18 | 6 |
| N6 | nd | Extraction blank | nd | nd | 6 | 6 | 1 |
| 37_b | nd | Extraction blank | nd | nd | 14 | 14 | 3 |
| 57_b | nd | Extraction blank | nd | nd | 20 | 20 | 6 |
| Pbl | nd | PCR negative | nd | nd | 0 | 0 | 0 |