Fig 3. DTV titer from infected male SG cultures.
Infectious virus release from Powassan virus lineage II (DTV)-infected male I. scapularis salivary gland (SG) cultures was quantified using immunofocus assays. Experimental and control samples were inoculated 5 x 105 focus-forming units of DTV. Immunofocus assays were performed using supernatants collected from experimental wells with SGs infected with DTV and control wells with DTV inoculum and no SGs (A). Error bars represent the standard error of the means. Viral titers per timepoint were averaged and the log10 values were calculated. Initial inoculum (control) logarithmic values were then subtracted from the corresponding infectious virus release logarithmic values per timepoint to visualize timepoints of peak virus release (B). Data is representative of 3–4 biological replicates and 2 technical replicates per timepoint. Data were processed using GraphPad Prism software v7.04.