Fig 5. Localization of LGTV^GFP in infected SG cultures from male ticks.

Whole mounts of mock- and green fluorescent protein tagged-Langat virus (LGTV^GFP)-infected salivary glands (SGs) from male Ixodes scapularis ticks were imaged via confocal microscopy. Each image displays a single SG from a pool of 3 SG pairs per experimental group. A-D images were taken at 40X and insert E was taken at 63X magnification, representing the portion of image D outlined with the red box. Image processing was consistent among respective timepoints. LGTV^GFP-infected SGs shows both discrete puncta as well as broad aggregations of GFP expression in granular acini. Red arrows indicate LGTV^GFP, blue arrows indicate autofluorescence, and white arrows indicate DAPI stained cell nuclei. Samples from mock-infected organs were shown in order to compare SG autofluorescence.