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. 2020 Oct 15;15(10):e0240659. doi: 10.1371/journal.pone.0240659

Fig 6. Co-localization of CE labeled LDL with neutral -stained LDs.

Fig 6

HeLa cells were plated at 30% confluency on a glass cover slips and pre-incubated in serum free media for 24 hours prior to experiments. Labelled LDL were added to the media for 1 hour, washed and chased for 60 minutes in serum/lipoprotein free media. Cells were stained for LDs utilizing Neutral BODIPY as described in Materials and methods. The confocal images of -CE labeled LDL co-localized with neutral -stained LDs are demonstrated for various receptor expressing HeLa cells. The color code and extent of co-localization are shown in the right columns. The cell type is specified at the top of the figure. The bar corresponds to 10 μm, * p < 0.001.