Table 1. Pulldown-mass spectrometric analysis reveals CEBPB, CEBPD and CCDC6 as binding partners for DN-ATF5.
PC3 cells were transfected with GFP-FLAG-DN-ATF5 or GFP and two days later cell lysates were prepared, incubated with anti-FLAG a beads and the beads eluted with 3x-FLAG peptide. The eluates were subjected to mass spectrometry. Data from two independent experiments are presented. Table includes examples of 5 abundantly recovered proteins in the eluates
| PROTEIN ID | EXPT 1 SPECTRAL COUNTS GFP | EXPT 1 SPECTRAL COUNTS DN-ATF5 | EXPT 2 SPECTRAL COUNTS GFP | EXPT 2 SPECTRAL COUNTS DN-ATF5 | EXPT 2 LFQ INTENSITY DN-ATF5/GFP | ||
|---|---|---|---|---|---|---|---|
| DN-ATF5 | 0 | 411 | 22 | 415 | 88.1 | ||
| CEBPB | 0 | 20 | 7 | 23 | 11.1 | ||
| CEBPD | 0 | 3 | 1 | 4 | 10.7 | ||
| CCDC6 | 0 | 9 | 0 | 19 | 68.1 | ||
| EIF4B | 35 | 50 | 50 | 20 | 0.65 | ||
| STK38 | 38 | 40 | 75 | 36 | 0.52 | ||
| PPM1B | 18 | 36 | 15 | 6 | 0.79 | ||
| FLNA | 6 | 20 | 47 | 17 | 0.54 | ||
| PLEC | 5 | 39 | 35 | 15 | 0.79 |