Author response image 1. Rotifer consumption quantification in larvae with different food experience levels.

(A) Example images of gut food fluorescence signal (magenta) from animals fed live rotifers stained with TexasRed at 6 dpf for 30 minutes. We analysed data from larvae which were previously introduced to live prey (unstained rotifers) at 5 dpf (1 day food experience) versus more experienced animals fed at 4&5 dpf (2 days food experience). Bottom pannels display TexasRed signal, dotted lines demarcate the gut area used to quantify fluorescence intensity. (B) Gut fluorescence quantification of food ingested in a 30 minute period. Coloured symbols denote the fluorescence level from example images. Error bars represent means ± SD.