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. 2020 Oct 5;9:e60259. doi: 10.7554/eLife.60259

Figure 2. Met/SAM cycle perturbations activate B12-mechanism-II.

Figure 2.

(A) Workflow for the EMS mutagenesis screen using Pacdh-1::GFP;∆nhr-10 reporter animals supplemented with 20 nM vitamin B12. WGS – whole genome sequencing. (B) To-scale cartoons of amino acid changes in the proteins encoded by the genes found in the forward genetic screen. (C) Workflow for RNAi screen using Pacdh-1::GFP;∆nhr-10 reporter animals supplemented with 20 nM vitamin B12. (D) Fluorescence microscopy images of Pacdh-1::GFP;∆nhr-10 reporter animals subjected to RNAi of the indicated metabolic genes. Insets show brightfield images. (E) Cartoon illustrating the activation of B12-mechanism-II by low vitamin B12 or genetic perturbations in the Met/SAM cycle.