Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 30;11:567191. doi: 10.3389/fgene.2020.567191

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Aga, Hallahan, Gottmann, Jaehnert, Osburg, Schulze, Kamitz, Arends, Brockmann, Schallschmidt, Lebek, Chadt, Al-Hasani, Joost, Schürmann and Vogel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Combined approach of haplotype and gene expression analysis in islets for the identification of DBA-specific gene variants within the Nidd/DBA locus. (A) Generation of congenic mice carrying different fragments of the QTL Nidd/DBA. (B) Diabetes prevalence (blood glucose >16.6 mM, week 16), (C) body weight, and blood glucose development in congenic mice carrying one heterozygous DBA allele for the Nidd/DBA locus compared to homozygous NZO controls. (D) Haplotype map of single nucleotide polymorphisms (SNPs) within the critical region of Nidd/DBA (108.8–111.4 Mbp). Black line: total number of SNPs (all SNPs annotated for the B6 reference genome with calls for NZO and DBA). Red line: SNPs according to B6 = NZO ≠ DBA. (E) QTL was dissected into windows of 250 kb. Red boxes indicate 100 SNPs per window polymorphic according to B6 = NZO ≠ DBA. Location of genes within the critical Nidd/DBA interval with a differential expression in pancreatic islets of 6 weeks old congenic mice (N/N ≠ D/D) and position of genes with non-synonymous coding variants between the parental strains NZO and DBA (REL-1505-GRCm38). (F) Genes within the critical Nidd/DBA interval displaying a differential expression in pancreatic islets of congenic mice (RCS-I, Nidd/DBA.13.6). Expression was analyzed via RNA-sequencing (n = 4). Genes with differential expression in pancreatic islets and location within a polymorphic Nidd/DBA haplotype block are indicated in red. Data are presented as means ± SEM and were analyzed with Student’s t-test (F) or one-way ANOVA (C). ^*p < 0.05; ^**p < 0.01; ^***p < 0.001.