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. 2020 Sep 30;11:575488. doi: 10.3389/fimmu.2020.575488

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Copyright © 2020 Cruz-Barrera, Flórez-Zapata, Lemus-Diaz, Medina, Galindo, González-Acero, Correa, Camacho, Gruber and Salguero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of immune modulatory secretome in UC-MSC/PBMNC cocultures. Global differences in cytokine concentrations in supernatants from UC-MSC/PBMNC as compared to activated and resting PBMNC in cell-contact and transwell experimental settings were visualized by unsupervised hierarchical clustering in (A) PHA-stimulated and (B) αCD3/CD28-stimulated UC-MSC/PBMNC cocultures. (C) Two-dimensional Principal Component Analyses (PCA) was carried out to determine associations between expression patterns of analyzed factors in PHA and αCD3/CD28 cocultures. (D) Radial visualization (RadViz) analyses showing factors assigned to dimensional anchors placed on the circumference of each circle for PHA and αCD3/CD28 stimulations in cell contact and transwell formats.