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. 2020 Sep 30;11:552818. doi: 10.3389/fphar.2020.552818

Figure 3.

Figure 3

Effects of NF-κB inhibition on the activation of fibroblasts. Cell viability and protein expression were measured in NIH-3T3 fibroblasts after their treatment with recombinant DPP4 (200 ng/ml) in the presence or absence of Bay11-7082 (1 µM), a NF-κB inhibitor. (A) Cell viability of fibroblasts exposed to Bay11-7082. (B) Protein expression determined using Western blotting. Calculated ratios of (C) p-p65 to GAPDH, (D) collagen I to GAPDH, (E) elastin to GAPDH, and (F) α-SMA to GAPDH were shown. (n = 4) *p < 0.05 vs. control. # p < 0.05 vs. DPP4.