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. 2020 Sep 30;11:552818. doi: 10.3389/fphar.2020.552818

Figure 4.

Figure 4

Effects of PAR2 antagonist or DPP4 inhibition on the activation of fibroblasts. Cell viability and protein expression measured in NIH-3T3 fibroblasts after their treatment with recombinant DPP4 (200 ng/ml) in the presence or absence of GB83 (10 µM) or linagliptin (30 nM). (A) Cell viability of fibroblasts exposed to GB83. (B) Protein expression determined using Western blotting. Calculated ratios of (C) collagen I to GAPDH, (D) elastin to GAPDH, (E) α-SMA to GAPDH, (F) NF-κB p-p65 to GAPDH, and (G) p-SMAD2/3 to GAPDH were shown. (n = 4) (H) Immunofluorescent staining of α-SMA. *p < 0.05 vs. control. # p < 0.05 vs. DPP4.