Table 1.
Sense e antisense primers sequences used for real-time quantitative PCR.
| Gene | 5′ Primer (5′-3′)-Sense | 3′ Primer (5′-3′)-Antisense |
|---|---|---|
| Gapdh | CCACCACCCTGTTGCTGTAG | CTTGGGCTACACTGAGGACC |
| Tfam | GGAATGTGGAGCGTGCTAAAA | TGCTGGAAAAACACTTCGGAATA |
| Nrf1 | CGCAGCACCTTTGGAGAA | CCCGACCTGTGGAATACTTG |
| Ucp1 | ACTGCCACACCTCCAGTCATT | CTTTGCCTCACTCAGGATTGG |
| Pgc1-α | ATCTACTGCCTGGGGACCTT | ATGTGTCGCCTTCTTGCTCT |
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG |
| Atgl | GGTCCTCTGCATCCCTCCTT | CTGTCCTGAGGGAGATGTC |
| Hsl | GGGAGGGCCTCAGCGTTCTCACA | ATAGCACGGAGCTGGGTGAGGG |
| Perilipin | AGTGTGGGGTCCTTGGGCGT | TGGCAGCTGTGAACTGGGTGG |
| Lpl | GGCCAGATTCATCAACTGGAT | GCTCCAAGGCTGTACCCTAAG |
| Fabp4 | AAGGTGAAGAGCATCATAACCCT | TCACGCCTTTCATAACACATTCC |
| Lipin | TGATGTGGTGTTCAGTGTCACT | TCGTTGACCCAGTGCAGGTA |
| Dgat1 | GGCCTGCCCCATGCGTGATTAT | CCCCACTGACCTTCTTCCCTGTAGA |
| Dgat2 | GAAGCTGCCCGCAGCGAAAA | TCTTGGGCGTGTTCCAGTCAA |
| Fasn | AGAGGCTTGTGCTGACTTCC | GTGGCTTCGGCGATGAGAG |
| Acc1 | GAGAGGGGTCAAGTCCTTCC | ACATCCACTTCCACACACGA |
| Glut-4 | CATTCCCTGGTTCATTGTGG | GAAGACGTAAGGACCCATAGC |
| Leptin | CATCTGCTGGCCTTCTCCAA | ATCCAGGCTCTCTGGCTTCTG |
| Adipoq | GCAGAGATGGCACTCCTGGA | CCCTTCAGCTCCTGTCATTCC |
| C/EBP-α | CGCAAGAGCCGAGATAAAGC | CAGTTCACGGCTCAGCTGTTC |
| Ppar-γ2 | GCATCAGGCTTCCACTATGGA | AAGGCACTTCTGAAACCGACA |
| Mcp1 | GCCCCACTCACCTGCTGCTACT | CCTGCTGCTGGTGATCCTCTTGT |
| Tnf-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| Pref-1 | GACCTGGAGAAAGGCCAGTA | AGGGAGAACCATTGATCACG |
| C/EBP-β | GCA AGA GCC GCG ACA AG | GGC TCG GGC AGC TGC TT |
| Cpt1 | TGTCCAAGTATCTGGCAGTCG | CATAGCCGTCATCAGCAACC |
| Nos2 | GCCACCAACAATGGCAACA | CGTACCGGATGAGCTGTGAATT |
| Gpx | CAGGAGAATGGCAAGAATGAAG | GAAGGTAAAGAGCGGGTGAG |
| Cat | GCAGATACCTGTGAACTGTC | GTAGAATGTCCGCACCTGAG |
| Sod1 | AAGACTGGAAATGCTGGGAG | GGTTTGAGGGTAGCAGATGAG |
| Sod2 | TGCTCTAATCAGGACCCATTG | CATTCTCCCAGTTGATTACATTCC |
Gapdh, Glyceraldehyde-3-phosphate Dehydrogenase; Tfam, Transcription Factor A, Mitochondrial; Nrf1, Nuclear Respiratory Factor 1; Ucp1, Uncoupler protein 1; Pgc1-α: Peroxisome Proliferator-Activated Receptor Gamma Coativator 1-alpha; Prdm16, PR-domain containing 16; Atgl, adipocyte triacylglycerol lipase; Hsl, hormone sensitive lipase; Lpl, Lipoprotein lipase; Fabp4, Fatty Acid Binding Protein 4; Dgat1, Diacylglycerol acyltransferase 1; Dgat2, Diacylglycerol acyltransferase 1; Fasn, fatty acid sinthase; Acc1, Acetyl-CoA carboxylase; Glut-4, glucose transporter 4; Adipoq, adiponectin; C/EBP-α, CCAAT/enhancer-binding protein alpha; Ppar-γ2, Peroxisome proliferator-activated receptor gamma; Mcp1, monocyte chemoattractant-1; Tnf-α, Tumor necrosis factor alpha; Pref-1, preadipocyte factor 1; C/EBP-β, CCAAT/enhancer-binding protein beta; Cpt1, carnitine palmitoyltransferase 1; Nos2, nitric oxide synthase 2; Gpx, glutathione peroxidase; Cat, Catalase; Sod1, superoxide dismutase 1; Sod2, superoxide dismutase 2.