Table 2.
Nucleic acid-based diagnostics of SVA infection developed in China.
| Diagnostics | Target sequence | Limit of detection | No cross-reaction with | References |
|---|---|---|---|---|
| RT-PCR | 5′ UTR | 1 TCID50/mL | EMCV, FMDV, PRV, PRRSV, CSFV, and PEDV | (57) |
| qRT-PCR | VP1 | 10 copy/μL | VSV, APPV, PRV, and FMDV | (58) |
| qRT-PCR | 3D | 10.4 copy/μL | FMDV, PRRSV, PEDV, PCV2, and PRV | (24) |
| RPA | 3D | 28 copy/μL | FMDV, CSFV, PRRSV, PEDV, and TGEV | (63) |
| RT-LAMP | VP1 and VP2 | 1 TCID50/mL | PCV2, PEDV, CSFV, PRRSV, PRV, VSV, SVDV, and FMDV | (60) |
| RT-ddPCR | 3D | 1.53 ± 0.22 copy/reaction | FMDV, VSV, SVDV, PRRSV, CSFV, and PCV2 | (59) |
| RT-LAMP-LFD | 3D | 11 copy/μL | FMDV, SVDV, VSV, PDCoV, PEDV, TGEV, PRRSV and CSFV | (61) |
| RPA-LFD | 3D | 56 copy/μL | FMDV, CSFV, PRRSV, PEDV, TGEV, PRV, and PCV | (62) |
qRT-PCR, quantitative RT-PCR; RPA, recombinase polymerase amplification; RT-LAMP, reverse transcription loop-mediated isothermal amplification; RT-ddPCR, RT-droplet digital PCR; RT-LAMP-LFD, reverse transcription loop-mediated isothermal amplification-lateral flow dipstick; RPA-LFD, recombinase polymerase amplification-lateral flow dipstick; EMCV, encephalomyocarditis virus; FMDV, foot-and-mouth disease virus; PRV, pseudorabies virus; PRRSV, porcine reproductive and respiratory syndrome virus; CSFV, classical swine fever virus; PEDV, porcine epidemic diarrhea virus; VSV, vesicular stomatitis virus; APPV, atypical porcine pestivirus; PCV2, porcine circovirus 2; TGEV, transmissible gastroenteritis virus; SVDV, swine vesicular disease virus; PDCoV, porcine deltacoronavirus.