Figure 5.

Breast cancer cells continuously treated with tamoxifen overexpress the kinin B1R, but not ERα. MCF-7 cells that were under treatment with tamoxifen for 7 days together with their respective untreated controls were stimulated with 1,000 nM tamoxifen by 24, 48, and 72 h. Cell proteins were separated by SDS-PAGE, transferred onto Immobilon-P and immunoblotted with antibodies for detection of ERα (A) and kinin B1R (B). Antibodies were stripped off and the same membranes were incubated with an antibody directed to GAPDH as control for protein loading. Data are representative of two independent experiments (n = 2). *P < 0.05; **P < 0.001 between tamoxifen-treated and untreated cells.