Fig. 1.

SPAs are required for thermomorphogenesis. (A) Photograph showing seedling phenotypes of spa higher order mutants grown at normal or high ambient temperature. Seedlings were grown for 2 days in continuous white light at 22°C and then either kept at 22°C or transferred to 28°C for an additional 4 days before being photographed. More than 10 seedlings were measured for each experiment, and the experiment was repeated three times for one-way ANOVA with Tukey's HSD test. Scale bar: 2 mm. (B) Bar graph shows the hypocotyl lengths for seedlings grown in the conditions described in A. The letters a-f indicate statistically significant differences between means of hypocotyl lengths (P<0.05) based on one-way ANOVAs. Error bars indicate the s.d. (n=3). (C) Western blot shows the level of TAP-SPA1 from 35S:TAP-SPA1 whole seedlings grown for 5 days at 22°C and either kept at 22°C or transferred to 28°C for 4 h. TAP-SPA1 was detected using Myc antibody. The red numbers indicate the quantitation value from the anti-Myc band divided by the Coomassie Blue staining intensity. (D) Bar graph shows the relative amount of TAP-SPA1 (n=3).