Fig. 3.

The daf-2 mutant modifies the effects on lifespan of M. nematophilum strains. (A) Lifespan of N2 on M. nematophilum CBX102 under CGI was significantly reduced (TD₅₀=11 days) when compared to both the derived M. nematophilum UV336 strain and E. coli OP50 that produced identical TD₅₀ (19 days). (B) Lifespan of daf-2 on M. nematophilum CBX102 under CGI (TD₅₀=33) was statistically indistinguishable (P=0.4531) from OP50 (TD₅₀=36). In contrast, lifespan on UV336 was significantly increased (P<0.0001; TD₅₀=49). For experiments involving the temperature sensitive daf-2, lifespan assays started at day 0 when animals were age-synchronized by bleach. Embryos were then left at 15°C on the appropriate bacterial diet until day 5. Day 5 marks the L4-to-adult transition and the time when plates were transferred to 25°C. The graph is A is the same experiment as in Fig. 1A. All experiments shown in Figs 1 and 3 were conducted in parallel. N=25 per treatment. Results are from three independent experiments.