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. 2020 Oct 7;9(10):bio053504. doi: 10.1242/bio.053504

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — FOXO mediates the extension of daf-2 lifespan on CBX102 under CGI. The daf-2-mediated lifespan extension on CBX102 was suppressed by daf-16/FOXO, using two mutants (mu86 and mgDf47) of daf-16. We found that when compared to each other and to N2, daf-16, daf-2 double mutants and N2 had a lifespan with identical TD₅₀ (12 days) on CBX102. This was also the lifespan (TD₅₀) of daf-16(mu86) alone (12 days). In contrast, the lifespan of daf-2 on CBX102 under CGI was significantly different (TD₅₀=33, P<0.0001). For experiments involving the temperature sensitive daf-2, lifespan assays started at day 0 when animals were age-synchronized by bleach. Embryos were then left at 15°C on the appropriate bacterial diet until day 5. Day 5 marks the L4-to-adult transition and the time when plates were transferred to 25°C. N=25 per treatment. Results are from three independent experiments.