Figure 1.

Expression of SRSF1 Was Closely Related to Satellite Cell Activation and Proliferation

(A) Skeletal muscle protein samples were harvested from P1, P6, and P70 C57BL/6 mice and western blots were performed using the indicated antibodies (n = 3).

(B) Primary satellite cells were isolated from C57BL/6 mice at the age of P6. Cells were cultured in growth medium and stained with Myf5 (green) or SRSF1 (red). Right panels show merged images with DAPI (blue). Percentage of Myf5⁺ cells also marked with SRSF1 staining was shown on the right histogram. Scale bar, 100 μm. Data were shown as mean ± SEM in an independent experiment (n = 3).

(C) Extensor digitorum longus (EDL) myofibers were prepared from adult mice and cultured in vitro. Immunostaining was performed using antibodies against PAX7 (green), MyoD (green), MyHc (green), Ki67 (green), or SRSF1 (red) at indicated time. Bottom panels show merged images with DAPI (blue). Fraction of PAX7⁺, MyoD⁺, ki67⁺, or MyHc⁺ nuclei that are also marked by SRSF1 staining was shown in histogram on the right panel. Scale bars, 20 μm (0 h), 20 μm (12 and 72 h), or 50 μm (144 h), respectively. Data were shown as mean ± SEM in an independent experiment (n = 3).