Figure 5.
SRSF1 Deficiency Dramatically Impaired NMJ Maturation in the DIA Muscles
(A) Whole-mount DIA staining at P1 or P22. DIA prepared from SRSF1f/f (left) or MKO (right) at P1 or P22 were stained with synaptophysin (Syn, red) and α-BTX (green). Merged pictures are shown on the right. Scale bars, 50 μm for the P1 group or 100 μm for the P22 group.
(B) The number of merged NMJs and NMJ area width were measured between the P22 group described in (A) (n = 3). The mean value of the merged NMJ in SRSF1f/f mice was arbitrarily defined as 1.
(C) Enlarged view of white boxes in the merged panel of P1 DIA in (A). Scale bar, 15 μm.
(D) Enlarged view of blue boxes in the merged panel of P22 DIA in (A) (n = 3). Scale bar, 30 μm.