Figure 2.

The Mechanism Underlying Androgen's Effect on ISC Differentiation

Quantitative real-time PCR was performed to investigate the mRNA levels of differentiation-related factors. Data, indicated as mean ± SD, correspond to three independent experiments (n = 4 mice/group/experiment).

(A and B) For the goblet cell fate specification, the relative mRNA levels of (A) Klf4 and (B) Elf3 were detected in the ORX, OVX, and inhibition of AR models.

(C) For the Paneth cell fate specification, the relative mRNA levels of Sox9 were detected in the ORX, OVX, and inhibition of AR models.

(D) For the enteroendocrine cell fate specification, the relative mRNA levels of Ngn3 were detected in the ORX, OVX, and inhibition of AR models.

(E) For the secretory lineage specification, the relative mRNA levels of atoh1 were detected in the ORX, OVX, and inhibition of AR models.

(F) The expression of Atoh1 was assessed by western blot analysis.

^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001