Figure 5.

Androgen Regulated the BMP-Related Gene Expression in Intestinal Stromal Cells

(A) Indicated mRNAs were analyzed by quantitative real-time PCR. Values were normalized to Gapdh expression and expressed relative to each gene of the Wnt pathway.

(B) Quantitation of antagonists of the Wnt signal pathway.

(C and D) mRNA levels determined by quantitative real-time PCR of (C) Tgfb1 and Bmp4 and (D) antagonists of BMP. In (A), (B), (C), and (D), all the cDNAs used were synthesized from stromal cells, stromal cells with DHT, and stromal cells with DHT and flutamide for 4 h.

(E and F) Quantitation of (E) Cdkn1a, Cdkn2a, and Cdkn2b (TGF-β downstream genes) and (F) Id2, Id3, and Msx2 (BMP downstream genes). In (E) and (F), values are means + SEM. Data, indicated as mean ± SD, correspond to three independent experiments.

^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.