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. 2020 Sep 3;15(4):926–940. doi: 10.1016/j.stemcr.2020.08.002

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Proteome Analysis to Identify Metabolic Enzymes as Damaged-Myofiber-Derived Factors

(A) A schematic illustrating the proteome analysis. To distinguish damaged-myofiber-derived proteins from supernatant, MALDI-TOF/MS analysis was performed. Individual myofibers were freshly isolated from EDL muscles and mechanically damaged as shown in Figure 2. Supernatant from damaged myofibers was collected at 72 h following myofiber injury. Supernatant from intact myofibers was used as control.

(B and C) (B) Top six enriched proteins are listed. These enriched proteins were confirmed by their biological replication. (C) Proteins highlighted with red font are the metabolic enzymes that are involved in the pathways for glycolysis and production of ATP.