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. 2020 Sep 10;15(4):955–967. doi: 10.1016/j.stemcr.2020.08.005

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Reduced Tibia Length and Abnormal Tibial Epiphyseal Cartilage Organization in Prx1^Cre;Fgfr3^Y367C/+ Mice

(A) Three-month-old Prx1^Cre;Fgfr3^Y367C/+ and Prx1^Cre;Fgfr3^+/+ mice (top), radiographs of hindlimbs (bottom), and quantification of tibia length (right) (n = 5 per group). Scale bar, 0.5 cm.

(B) Representative Safranin O (SO) staining of epiphyseal cartilage of uninjured tibia from 1- to 3 month-old Prx1^Cre;Fgfr3^+/+ and Prx1^Cre;Fgfr3^Y367C/+ mice. Scale bar, 50 μm.

(C) Immunofluorescence of Collagen X (COLX), Osterix (OSX), and CD31/Endomucin (EMCN) at the transition zone between the epiphyseal hypertrophic cartilage (hc) and the metaphysis in uninjured tibia from 3 months old Prx1^Cre;Fgfr3^+/+ and Prx1^Cre;Fgfr3^Y367C/+ mice (n = 3). Scale bar, 50 μm.

(D) Quantification of CD31 and EMCN immunofluorescence at the transition zone between the epiphyseal cartilage and the metaphysis from Prx1^Cre;Fgfr3^+/+ and Prx1^Cre;Fgfr3^Y367C/+ mice (n = 6).

Values represent mean ± SD. ^∗∗p < 0.01 using Mann-Whitney test.